Gram – CSF: mononuclear cells – yeast elements (Cryptococcus) – Shipped February 2012
The sample was a simulated CSF obtained from a 35 year old HIV + man and was sent to participant laboratories for Gram staining. Participants were expected to report the presence of yeast elements and mononuclear cells.
MAIN EDUCATIONAL POINTS from GS114-1
- It is important that laboratories correctly differentiate and report the predominant WBC type in sterile site (especially CSF) samples as this assists in identifying the most likely etiologic agent.
- If laboratories are unable to distinguish between mononuclear (eg. lymphocytes) and polymorphonuclear (eg. neutrophils) cells on the Gram stain then they should report as white blood cells (WBCs) without type differentiation.