Gram: Corneal scraping: no cells –2+ (2-10/oif) gram positive bacilli (Bacillus cereus) – Shipped August 09, 2021
A simulated corneal scraping sample collected from a 37 year old male stone worker with eye injury was sent to category A and C1 laboratories. Participants were expected to report gram positive bacilli and no cells.
MAIN EDUCATIONAL POINTS from G212
- Gram staining procedures should be reviewed for the laboratories that reported gram negative bacilli. Caution should be taken to not over decolorize the slides when staining. A Gram stain control might be helpful for comparison of organisms and staining.
- Bacillus infections can be serious and result in total loss of vision with enucleation.