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G212

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Gram: Corneal scraping: no cells –2+ (2-10/oif) gram positive bacilli (Bacillus cereus) – Shipped August 09, 2021

HISTORY

A simulated corneal scraping sample collected from a 37 year old male stone worker with eye injury was sent to category A and C1 laboratories. Participants were expected to report gram positive bacilli and no cells.

MAIN EDUCATIONAL POINTS from G212

  1. Gram staining procedures should be reviewed for the laboratories that reported gram negative bacilli. Caution should be taken to not over decolorize the slides when staining. A Gram stain control might be helpful for comparison of organisms and staining.
  2. Bacillus infections can be serious and result in total loss of vision with enucleation.

Full critique (PDF)

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