M143-5

Blood culture: Leptotrichia buccalis – Educational challenge  – Shipped November 2014

HISTORY

The challenge was sent to category A laboratories. The sample was a simulated blood culture obtained from an 18 year old, male patient with febrile neutropenia.

Participants were expected to isolate and identify Leptotrichia buccalis.

MAIN EDUCATIONAL POINTS from M143-5

  1. Leptotrichia is a gram negative, fusiform, commensal oral anaerobe that is an emerging pathogen, particularly in immunocompromised hosts.
  2. Leptotrichia may be misidentified as Fusobacterium, Lactobacillus, or Capnocytophaga without the aid of molecular methods, due to a propensity of young cultures to retain Gram stain, and old cultures to grow in CO2.

Full critique (PDF)

Posted in Clinical Bacteriology Results, critiques