Gram – Joint Fluid: neutrophils and gram positive cocci (Group A Streptococcus) – Shipped
A smear from a simulated joint fluid collected from a 5 year old female with severe sepsis was sent to category A, B, C, and C1 laboratories for Gram staining.
Participants were expected to report the presence of gram positive cocci and neutrophils.
MAIN EDUCATIONAL POINTS from GS132-1
- The key aspect of this challenge is the ability to recognize WBC/leukocytes in a joint fluid aspirate and to identify and report the presence of gram positive cocci.
- If typical morphology is demonstrated, suggesting a bacterium’s genus is very valuable preliminary information. However, laboratories are cautioned not to overcall if the morphology is not characteristic.