GS114-1

Gram – CSF: mononuclear cells yeast elements (Cryptococcus) – Shipped February 2012

HISTORY

The sample was a simulated CSF obtained from a 35 year old HIV + man and was sent to participant laboratories for Gram staining. Participants were expected to report the presence of yeast elements and mononuclear cells.

MAIN EDUCATIONAL POINTS from GS114-1

  1. It is important that laboratories correctly differentiate and report the predominant WBC type in sterile site (especially CSF) samples as this assists in identifying the most likely etiologic agent.
  2. If laboratories are unable to distinguish between mononuclear (eg. lymphocytes) and polymorphonuclear (eg. neutrophils) cells on the Gram stain then they should report as white blood cells (WBCs) without type differentiation.

 

Full critique (PDF)

Posted in Clinical Bacteriology Results, critiques, Gram