G182

Gram – Joint Fluid: no neutrophils, 4+ (>50/oif) gram positive bacilli (Clostridium perfringens) – Shipped August 13, 2018

HISTORY

A simulated joint fluid sample collected from a 27 old athlete, post arthroscopy on knee was sent to category A, C, and C1 laboratories. Participants were expected to note the absence of cells and the presence of gram positive bacilli

 

MAIN EDUCATIONAL POINTS from G182

  1. Gram smears from tissue or fluid samples from patients infected with C. perfringens may typically have no or very few neutrophils present.
  2. The morphology of C. perfringens in tissue or fluid samples is one of large, boxcar-shaped bacilli. Some bacterial cells in the smear may tend to lose their Gram reaction.

Full critique (PDF)

Posted in Clinical Bacteriology Results, critiques, Gram