G114

Gram – Central line wound: Gram positive bacilli. results cells results bacteria – Shipped February 2012

HISTORY

The sample was a simulated central line wound sample from a 50 year old man with leukemia, bacteremia, and on anti-leukemic therapy. The challenge was sent to category A, B, C, and C1 laboratories for Gram staining.

Participants were expected to report the presence of gram positive bacilli.

MAIN EDUCATIONAL POINTS from G114

  1. Cellular components that are not present in a Gram stain should not be reported including neutrophils, epithelial cells and red blood cells. Laboratories that reported the presence or either neutrophils or epithelial cells should review their slides.
  2. Corynebacterium may be pleomorphic on Gram stain and morphologies may include bacilli, and coccobacilli.

 

Full critique (PDF)

Posted in Clinical Bacteriology Results, critiques, Gram