G153

Gram – Wound: gram negative bacilli, neutrophils — Companion to M153-3 – Shipped 02 November 2015

HISTORY

A simulated wound smear collected from a 36 year old male, returning traveler from Thailand, was sent to category A, B, C, and C1 laboratories for Gram staining.

Participants were expected to report the presence of neutrophils and gram negative bacilli.

MAIN EDUCATIONAL POINTS from G153

  1. Neutrophils are commonly observed in Gram stains, especially from infected wounds. Their presence is an indicator of an active inflammatory process.
  2. The Gram stain of Acinetobacter species shows gram negative bacilli or coccobacilli, that are fat and relatively large. They do underdecolorize and may appear coccoid; however, they should not be mistaken for gram positive organisms or called diplococci.

Full critique (PDF)

 

Posted in Clinical Bacteriology Results, critiques, Gram Tagged with: , ,